Whole-plant hydraulic conductance and root-to-shoot flow of abscisic acid are independently affected by water stress in grapevines

Abstract

In order to investigate whether plant hydraulic conductance (gplant) is reduced under drought conditions via an ABA-related mechanism, a water-stress experiment was carried out using split-rooted grapevines. In addition, inversion of shoot growth orientation was imposed to reduce gplant independently of soil water availability, and thus of the putative ABA root-generated stress message. As expected, water stress imposed on split-roots affected ABA accumulation. ABA drought-stress message negatively affected stomatal conductance (gs) and transpiration (E), but modified neither leaf or stem water potentials (Ψleaf and Ψstem, respectively), nor gplant. When gplant was reduced in split-rooted, shoot-inverted (s-r/s-i) grapevines, Ψleaf and Ψstem decreased, without changes in ABA accumulation, gs and E. The ABA drought-stress message did not modify gplant, nor did gplant (impaired by shoot-growth inversion) decrease ABA delivery to the leaves. However, leaf growth was depressed in s-r/s-i grapevines. The fact that no interaction between ABA stress messages (caused by split-root technique) and hydraulic constraints to sap flow (caused by shoot inversion) was necessary to impair leaf growth suggests that the targets of ABA and hydraulic-limitation effects on leaf expansion are not the same.