Abstract
The possibility that a gene conditioning differences in osmoregulation in
wheat leaves (or) is also expressed in pollen grains was
investigated in order to differentiate heterozygous and homozygous lines for
use in backcross breeding. The difference in osmoregulation in leaves was
expressed as a difference in solute accumulation which was calculated from
responses of osmotic potential and relative water content to reductions in
water potential caused by droughting plants in pots. A corresponding allelic
difference was found in pollen grains. It was demonstrated by a difference in
relative size, and solute accumulation, after stressing using polyethelene
glycol solution. The response occurred only after addition of a small quantity
of potassium chloride, and the maximum response occurred at concentrations of
approximately ≥0.2 mM (at least to 10 mM). This suggests that
or may condition high affinity potassium transport.
Single-gene control was confirmed using pollen responses, and pollen grain
expression was further demonstrated by the use of pollen responses, to
identify alleles and thus manipulate leaf responses in a backcross breeding
study. Because the differences in pollen grain size may be readily observed
without measurement using a microscope with relatively low magnification, and
because the pollen grains are easily sampled at flowering, the pollen response
is suitable as a routine test in plant breeding involving pedigree or
backcross methods.
Subject
General Agricultural and Biological Sciences
Cited by
26 articles.
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