Rapid spot test for glutathione peroxidase activity: comparison with a spectrophotometric procedure and assessment of the test as a measure of selenium in the blood of grazing ruminants

Author:

Langlands JP,Donald GE,Bowles JE,Smith AJ

Abstract

The activity of the seleno-enzyme glutathione peroxidase in ovine and bovine blood was measured by using spot and spectropholometric assays, and was used to predict the selenium content of blood. Sources of error were examined in three experiments. In the first, the time from sampling and temperature at which samples were stored, the quantity of heparin used, the temperature of the assay and exercise of the sheep immediately preceding sampling were varied. In the second, relationships were calculated between blood selenium content and glutathione peroxidase activity assayed by the spot test and with a spectrophotometer. In the final experiment sources of variation in the spot assay were examined as affected by variation between animals, operators and days. Changes in glutathione peroxidase activity with time following sampling were small, and activities of samples stored at 3� and -28�C were similar; freezing samples in liquid nitrogen reduced the activity, which was also sensitive to the temperature of the assay. Exercise and the quantity of heparin used did not affect activity. Allometric relationships were calculated between blood selenium content and glutathione peroxidase activities determined by both spot and spectrophotometric assays. The latter was more precise. Differences between operators and between ewes and wethers in enzyme activity were not significant; variation between animals in blood selenium content increased with mean blood selenium content. It was concluded that the spot test would be satisfactory for identifying animals which could respond to selenium supplementation and would be particularly useful when equipment was not available for automated selenium determinations.

Publisher

CSIRO Publishing

Subject

General Agricultural and Biological Sciences

Cited by 9 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3