Cellular Location and Concentration of Leghaemoglobin in Soybean Root Nodules

Abstract

The reaction of leghaemoglobin (Lb) with oxidized 3,3'-diaminobenzidine has been used to demonstrate the localization of this haemoprotein within the membrane envelopes surrounding the bacteroids in soybean root nodule cells. In fresh tissue sections mounted in a modified Honda medium, the reagent stained the envelope contents a brick-red colour.