Author:
Pelton T. A.,Bettess M. D.,Lake J.,Rathjen J.,Rathjen P. D.
Abstract
Early mammalian embryogenesis is characterised by the coordinated
proliferation, differentiation, migration and apoptosis of a pluripotent cell
pool that is able to give rise to extraembryonic lineages and all the cell
types of the embryo proper. These cells retain pluripotent differentiation
capability, defined in this paper as the ability to form all cell types of the
embryo and adult, until differentiation into the three embryonic germ layers
at gastrulation. Our understanding of pluripotent cell biology and molecular
regulation has been hampered by the difficulties associated with experimental
manipulation of these cells in vivo. However, a more
detailed understanding of pluripotent cell behaviour is emerging from the
application of molecular technologies to early mouse embryogenesis. The
construction of mouse mutants by gene targeting, mapping of gene expression
in vivo, and modelling of cell decisions
in vitro are providing insight into the cellular origin,
identity and action of key developmental regulators, and the nature of
pluripotent cells themselves. In this review we discuss the properties of
early embryonic pluripotent cells in vitro and
in vivo, focusing on progression from inner cell mass
(ICM) cells in the blastocyst to the onset of gastrulation.
Subject
Developmental Biology,Endocrinology,Genetics,Molecular Biology,Animal Science and Zoology,Reproductive Medicine,Biotechnology
Cited by
24 articles.
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