Author:
Jawerbaum Alicia,Gonzalez Elida T.,Sinner Débora,Pustovrh Carolina,White Verónica,Gimeno Martha A. F.
Abstract
Diminished PGE2 levels in diabetic embryos are related
to the development of malformations, and thus the aim of the present study was
to determine whether PGE2 levels are modified in rat
embryos cultured in diabetic serum during organogenesis, and if
PGE2 content and release, and
3H-PGE2 uptake and release, are
altered in incubated diabetic embryos. Rats were made diabetic by
steptozotocin (60 mg kg–1) before mating. Control
rat embryos cultured for 24 h (explantation Day 9) in the presence of diabetic
serum showed diminished PGE2 levels. When Day 10
diabetic embryos were incubated, embryo PGE2 levels were
lower, but the PGE2 released to the incubation media was
much higher than in controls. Uptake of
3H-PGE2 by diabetic embryos was
initially enhanced (5–10 min), then reached similar levels to controls
(20–100 min). Release of
3H-PGE2 previously incorporated
during a 60-min incubation was greater in diabetic embryos than in controls.
These results show diminished PGE2 content in both
diabetic and normal embryos cultured in the presence of diabetic serum, but
suggest that diabetic embryos have the capability to produce and release high
levels of PGE2. The enhanced release of
PGE2 is probably the result of transport abnormalities,
and leads to the elevated PGE2 concentrations found in
the incubating medium and to the diminished intraembryonic
PGE2 levels that alter embryonic development.
Subject
Developmental Biology,Endocrinology,Genetics,Molecular Biology,Animal Science and Zoology,Reproductive Medicine,Biotechnology
Cited by
15 articles.
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