Increasing sucrose accumulation in sugarcane by manipulating leaf extension and photosynthesis with irrigation

Abstract

High sucrose content (SC) in sugarcane stalks is a priority for all sugarcane industries world wide. Partitioning to sucrose in the cane stalk is related to the supply of photo-assimilate and the demand for assimilate by other organs. If photosynthesis could be maintained, but leaf and stalk growth constrained, by genetics or management during the stalk elongation phase, it may be possible to reduce stalk height and to increase both SC and sucrose yield. This paper reports an experiment designed to test this hypothesis and to develop a methodology to assess variation in response to source–sink manipulation in sugarcane clones. The research was conducted on a ‘low’ (Q138) and a ‘high’ (Q183) SC cultivar in two temperature controlled and airtight glasshouses (chambers) at CSIRO’s Davies Laboratory in Townsville, Australia. Potted plants of each cultivar were placed in two chambers of the Tall Plant Facility (TPF). In one chamber, plants were irrigated to minimise water stress while plants in the other chamber were irrigated to reduce plant extension rate (PER) considerably more than photosynthesis. Water stress reduced gain in total biomass by 19% and gain in top mass by 37%, and increased sucrose mass gain by 27%. During the experiment, SC of dry matter increased 37% in the dry treatment and only 8% in the wet treatment and this effect was greater in Q183 than in Q138. Water stress reduced whole plant photosynthesis by 18%, thus largely accounting for the 19% reduction in biomass accumulation and it reduced PER by 41%, corresponding to the 37% reduction in mass of tops. Reduced PER resulted in reduced demand for photo-assimilate by fibre and tops thus allowing excess assimilate to accumulate in the form of sucrose. The techniques developed here to control PER and measure the resulting changes in carbon partitioning now allow further examination of both the control of the balance between growth and sucrose storage and the extent of genotypic variation to the response of reduced PER.