Author:
Nikolova Diana B.,Martinova Yordanka S.,Seidensticker Martin,Bellvé Anthony R.
Abstract
The effect of leukaemia inhibitory factor (LIF) on the proliferation of
prospermatogonial stem cells was testedin vitro. Pieces
of 3-day-old rat testis were cultured in the presence of a range of doses of
LIF alone or in combination with 1 ng mL -1 seminiferous
growth factor (SGF). Stimulation of the proliferative activity of quiescent
prospermatogonia was detected immunocytochemically with a cell proliferation
kit. After 24 h culture, LIF signicantly increased the percentage of labelled
prospermatogonia, with the peak of activity at 20 pg mL
-1 . The combination of LIF and SGF resulted in a
decrease in DNA synthetic activity of the germ cells. Hence, LIF and SGF play
a role in local regulation at the onset of spermatogenesis in the rat testis.
Subject
Developmental Biology,Endocrinology,Genetics,Molecular Biology,Animal Science and Zoology,Reproductive Medicine,Biotechnology
Cited by
12 articles.
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