Extraction and Partial Purification of Mouse Uterine Alkaline Phosphatase-Reference-Cited by-同舟云学术

Extraction and Partial Purification of Mouse Uterine Alkaline Phosphatase

Published:1979 Issue:2 Volume:32 Page:153
ISSN:0004-9417
Container-title:Australian Journal of Biological Sciences
language:en
Short-container-title:Aust. Jnl. Of Bio. Sci.

Author:

Murdoch RN,Kay DJ,Capper WJ

Abstract

Alkaline phosphatase in uterine homogenates from day 7 pregnant mice was solubilized using 0�2 % (v/v) Triton X-100 and extracted with 20% (v/v) n-butanol. The procedure, which resulted in 182- fold purification, included ammonium sulfate precipitation, DEAE-cellulose anion exchange chromatography and Sephadex 0200 gel filtration.

Publisher

CSIRO Publishing

Subject

Developmental Biology,Endocrinology,Genetics,General Materials Science,Molecular Biology,Animal Science and Zoology,Reproductive Medicine,General Medicine,Biotechnology

Link

https://www.publish.csiro.au/BI/pdf/BI9790153

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