Effect of Flemingia macrophylla silage on in vitro fermentation characteristics and reduced methane production

Abstract

Fodder shrub, Flemingia macrophylla silage (FMS), was nutritionally evaluated for rumen fermentation characteristics using an in vitro gas production technique. Dietary treatments were imposed in a 2 × 7 factorial arrangement in a completely randomised design. The first factor was two ratios of roughage:concentrate (R:C at 60:40 and 40:60), and the second factor was seven levels of FMS supplementation at 0, 20, 40, 60, 80, 100 and 120 g/kg of dietary substrate. The results revealed that a R:C ratio at 40:60 increased the kinetics of gas production (P < 0.001). Moreover, supplementation of FMS increased in vitro degradability and the concentration of propionic acid (P < 0.001), whereas acetic acid concentration, protozoal population and methane production were reduced (P < 0.001). In conclusion, supplementation of FMS at 120 g/kg of dietary substrate improved rumen fermentation and subsequently reduced methane production. However, in vivo feeding trials using FMS should be conducted to gain more information for further implementations.