In vitro Growth and Differentiation of Epithelial Cells derived from Post-embryonic Hair Follicles

Abstract

Cell aggregates formed during the first 2 days of culture of cells derived from hair folIicle tissue of young rats. Aggregates occurred at an accelerated rate at higher cell densities, and contained a high proportion of epithelial cells although a variable proportion of esenchymal (fibroblast) cells were present. Citrulline was detected in the cultured cell proteins. Electrophoretic analysis of the proteins showed the presence of hair cortical keratin in the cultures, but these proteins were not synthesized during the culture period, in conflict with previous findings.