Role of the antioxidant system in the regulation of the chlorophyll biosynthesis pathway in the vascular plant Cucumis sativus

Abstract

In this study, the role of the antioxidant system has been examined in the regulation of the chlorophyll biosynthesis pathway in the vascular plant Cucumis sativus L. To generate reactive oxygen species (ROS), etiolated (E) and green (G) cucumber cotyledons were treated with methyl viologen (MV) or were exposed to high light (HL, 400–500 µE m–2 s–1). ROS generation was confirmed by measuring proline and H2O2 concentrations. With the effects of the MV- and HL-induced oxidative stress, it was observed that the chlorophyll biosynthesis pathway was severely affected in the HL-treated etiolated cotyledons (E-HL), MV-treated etiolated cotyledons (E-MV) and in MV-treated green cotyledons (G-MV) at 5-amino levulinic acid (ALA) as well as at protoporphyrin IX and Mg-protoporphyrin IX monomethyl ester levels. The antioxidant assays conducted showed that the ascorbate peroxidase (APX) activity had decreased in the E-HL, E-MV and G-MV cotyledons along with the levels of ascorbate and lutein. A decrease in the NADPH-dependent thioredoxin reductase (NTRC) was also observed in the MV-treated cotyledons with a significant impairment of the catalase activity in the E-HL cotyledons. Conversely, in the HL-treated green i.e. G-HL cotyledons, where the accumulation of H2O2 and the inhibition of chlorophyll biosynthesis were not observed, an increase in the levels of APX, NTRC, peroxiredoxin, ascorbate, glutathione and lutein was noted. Thus, the results obtained suggested that the antioxidant system could influence the flow of the chlorophyll biosynthesis pathway through maintaining the levels of H2O2.