Factors affecting ammonia-oxidising microorganisms and potential nitrification rates in southern Australian agricultural soils

Abstract

Ammonia-oxidising archaea (AOA) have recently been described as having an important role in soil nitrification. However, published data on factors which influence their distribution and their impact on a soil’s potential nitrification rates (PNR) are sparse, particularly compared with the amount of information available regarding ammonia-oxidising bacteria (AOB). This study had two aims. First, to investigate which environmental factors affect the AOA : AOB ratio in soils from two agricultural regions, and second, to explore whether the abundance of either AOA or AOB correlated with PNR. Samples were collected from 45 sites within the cropping regions of Western Australia and South Australia. Soils were tested for pH, NH4+/NO3–, organic carbon (C), total nitrogen (N), C : N ratio, PNR, and electrical conductivity. Climate data were obtained from the Queensland Climate Change Centre for Excellence SILO website. Abundances of AOA and AOB were measured using real-time PCR quantification of the gene encoding the ammonia monooxygenase enzyme (amoA). Multivariate statistical analysis was applied to assess correlations between PNR, soil properties, and abundance of AOA or AOB. In the majority samples AOA were present, but their abundance, and the AOA : AOB ratio, varied considerably between sites. Multivariate analysis showed that the distribution of AOA and AOB and the AOA : AOB ratio were strongly correlated with climatic and seasonal factors. Sites where samples were collected during dry, hot periods tended to be AOA-dominated, whereas samples collected during cool, wet periods tended to be AOB-dominated or have equal abundances of AOA and AOB. The PNRs were correlated with total N content, organic C content, and soil pH. There was no clear correlation between AOA or AOB and PNR. This study shows that both AOA and AOB are widespread in Western Australian and South Australian soils and their abundance and ratio are affected by climate and season. It also shows that PNR is more strongly influenced by soil fertility factors than by the AOA : AOB ratio.