Author:
Gillan L.,Skovgold K.,Watson P. F.,Maxwell W. M. C.,Evans G.
Abstract
Ewes in a synchronized oestrus were
inseminated (intrauterine) with fresh and frozen–thawed spermatozoa and
the spermatozoa were either recovered from each section of the reproductive
tract after the animal was killed (Experiments 1a and 1b) or after they were
voided from the cervix (Experiment 2). In Experiment 1a, only 1.2 0.27%
of the original inseminate was recovered. Placing a ligature at the base of
the uterine horn in Experiment 1b led to the recovery of 3.0 0.33% of
the original inseminate, located mainly in each uterine horn (33.1
5.48%), and each isthmic and ampullary region of the oviduct (2.9
5.48% and 4.0 5.48%, respectively). A higher proportion of
spermatozoa recovered from the isthmus were uncapacitated when observed by
chlortetracycline staining than those recovered from the uterus (26.4
1.92% and 15.6 1.92%, respectively,
P<0.05). Experiment 2 showed that large proportions
of spermatozoa were voided from the tract through the vagina, with similar
numbers of fresh and frozen–thawed spermatozoa lost from the tract.
However, frozen–thawed spermatozoa were lost at a faster rate than fresh
(P<0.05) and with a more advanced membrane state
(66.8 1.30% and 53.2 1.30% were acrosome reacted respectively;
P<0.001). Large numbers of recovered spermatozoa had
lost their tails, with frozen–thawed spermatozoa more susceptible to
tail loss than fresh spermatozoa (55.0 0.96% and 45.5 0.96%
respectively; P<0.05).
Subject
Developmental Biology,Endocrinology,Genetics,Molecular Biology,Animal Science and Zoology,Reproductive Medicine,Biotechnology
Cited by
23 articles.
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