Growth, ion content, gas exchange, and water relations of wheat genotypes differing in salt tolerances

Abstract

Although the mechanisms of salt tolerance in plants have received much attention for many years, genotypic differences influencing salt tolerance still remain uncertain. To investigate the key physiological factors associated with genotypic differences in salt tolerance of wheat and their relationship to salt stress, 13 wheat genotypes from Egypt, Australia, India, and Germany, that differ in their salt tolerances, were grown in a greenhouse in soils of 4 different salinity levels (control, 50, 100, and 150 mm NaCl). Relative growth rate (RGR), net assimilation rate (NAR), leaf area ratio (LAR), photosynthesis, chlorophyll content (SPAD value), and leaf water relations were measured at Days 45 and 60 after sowing. Mineral nutrient content in leaves and stems was determined at Day 45 and final harvest. Salinity reduced RGR, NAR, photosynthetic rate, stomatal conductance, water and osmotic potentials, and K+ and Ca2+ content in stems and leaves at all times, whereas it increased leaf respiration, and Na+ and Cl– content in leaves and stems. LAR was not affected by salinity and the effect of salinity on SPAD value was genotype-dependent. Growth of salt-tolerant genotypes (Sakha 8, Sakha 93, and Kharchia) was affected by salinity primarily due to a decline in photosynthetic capacity rather than a reduction in leaf area, whereas NAR was the more important factor in determining RGR of moderately tolerant and salt-sensitive genotypes. We conclude that Na+ and Cl– exclusion did not always reflect the salt tolerance, whereas K+ in the leaves and Ca2+ in the leaves and stems were closely associated with genotypic differences in salt tolerance among the 13 genotypes even at Day 45. Calcium content showed a greater difference in salt tolerance among the genotypes than did K+ content. The genotypic variation in salt tolerance was also observed for the parameters involved in photosynthesis, and water and osmotic potentials, but not for turgor pressure.