Transcriptome profiling to identify tepal cell enlargement and pigmentation genes and the function of LtEXLB1 in Lilium tsingtauense

Abstract

To understand the molecular mechanism underlying tepal development and pigmentation in Lilium tsingtauense Gilg, we performed whole-transcriptome profiles from closed buds at the greenish tepal stage (CBS), the full-bloom with un-horizontal tepal stage (UFS), and the completely opened bud with reflected tepal stage (RFS) of L. tsingtauense. More than 95699 transcripts were generated using a de novo assembly approach. Gene ontology and pathway analysis of the assembled transcripts revealed carbon metabolism is involved in tepal development and pigmentation. In total, 8171 differentially expression genes (DEGs) in three tepal stages were identified. Among these DEGs, ~994 genes putatively encoded transcription factors (TFs), whereas 693 putatively encoded protein kinases. Regarding hormone pathways, 51 DEGs involved in auxin biosynthesis and signalling and 10 DEGs involved in ethylene biosynthesis and signalling. We also isolated seven LtEXPANSINs, including four EXPAs, one EXPB, one EXLA and one EXLB. LtEXLB1 (GenBank: MN856627) was expressed at higher levels in UFS and RFS, compared with CBS. Silencing LtEXLB1 in leaf discs and tepals by virus-induced gene silencing significantly decreased cell expansion under rehydration conditions. Further analysis revealed that more cell numbers were existed in the abaxial and adaxial subepidermis in the silenced LtEXLB1 samples. As the first transcriptome of L. tsingtauense, the unigenes are a valuable resource for future studies on tepal development, and LtEXLB1 functions in cell expansion.