Author:
Caemmerer Susanne von,Ludwig Martha,Millgate Anthony,Farquhar Graham D.,Price Dean,Badger Murray,Furbank Robert T.
Abstract
We have measured the discrimination against 13C during
CO2 assimilation in
Flaveria bidentis wild type plants and in transgenic
Flaveria bidentis plants transformed
(1) with an antisense RNA construct targeted to the
nuclear encoded gene for the small subunit of Rubisco—these plants had
reduced amounts of Rubisco, decreased CO2 assimilation
rates and increased carbon isotope discrimination, which was also evident in
the carbon isotope discrimination of leaf dry matter; and
(2) transformed with the mature coding region of
carbonic anhydrase, CA, from tobacco (Nicotiana tabacum)
in the sense direction under the control of the cauliflower mosaic virus 35S
promoter—these plants had slightly increased CA activity in the
mesophyll as well as a 2–4-fold increase in CA activity in the
bundle-sheath cells. The introduction of tobacco CA manifested itself by a
reduction in CO2 assimilation rate and an increase in
carbon isotope discrimination. We suggest that the increased carbon isotope
discrimination is a result of increased bicarbonate leakage out of the bundle
sheath.
Subject
Plant Science,Agronomy and Crop Science
Cited by
37 articles.
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