Author:
Basini G.,Baratta M.,Ponderato N.,Bussolati S.,Tamanini C.
Abstract
Nitric oxide (NO) is an important intra- and intercellular messenger
controlling many biological processes. It is synthesized by NO synthases,
which have been found also in granulosa cells. The present study examined
whether NO is present in bovine follicular fluid and is produced by granulosa
cells in culture. Secondly, it aimed to determine if NO affects the main
parameters of granulosa cell function. The NO donor
S-nitroso-L-acetyl-penicillamine (10 –3 , 10
–4 , 10 –5 М) was
used to evaluate whether NO might influence steroidogenesis, proliferation and
apoptosis in bovine granulosa cells collected from follicles divided according
to their size in small (<5 mm) and large (>8 mm). The data demonstrate
the presence of NO in follicular fluid and its production by granulosa cells
in culture: the most active cells in producing NO are those from the small
follicles, as confirmed by the NO levels in follicular fluid. This study also
shows that NO donor significantly (P<0.001) inhibits
progesterone (P4) and oestradiol 17β (E2) production by the granulosa
cells from both kinds of follicle; moreover, the highest concentration of NO
donor significantly (P<0.001) inhibits DNA
fragmentation in all the cells whereas the lowest concentration stimulates
(P<0.001) cellular apoptosis only in granulosa cells
from large follicles. NO donor does not seem to modify cell proliferation.
Taken together these data lead point to NO as a local modulator of granulosa
cell function.
Subject
Developmental Biology,Endocrinology,Genetics,Molecular Biology,Animal Science and Zoology,Reproductive Medicine,Biotechnology
Cited by
63 articles.
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