Author:
Le Bris Manuel,Michaux-Ferrière Nicole,Jacob Yves,Poupet Alain,Barthe Philippe,Guigonis Jean-Marie,Page-Degivry Marie-Thérèse Le
Abstract
In vitro cultures showed that the proximal buds isolated
from a rose (Rosa hybrida L. cv. Ruidriko Vivaldi®)
stem were endodormant. Growth and a high percentage of bud break could be
observed when cultures were treated with fluridone, an inhibitor of carotenoid
synthesis. Flow cytometry determination of nuclear DNA content revealed that
cell cycle activity of endodormant buds was arrested in the G
1 phase. Upon culture, the large decrease in bud ABA
content was responsible for the progress from G1 to
G2 phase whatever the culture medium. However, in
control culture, neither cell division nor leaf primordium initiation could be
observed and cells appeared stably arrested in G2 . By
contrast, with fluridone, an additional ABA decrease was observed resulting
from an inhibition of its synthesis inside the bud. New leaf primordia were
initiated and many figures of mitosis could be observed, indicating that
intense activity of cell division occurred after DNA replication. Therefore,
the results indicate that, as long as ABA was synthesized inside the buds,
cell cycle was arrested in G2 phase and buds remained
dormant. Continued in situ ABA biosynthesis appears, therefore, to be required
for the maintenance of bud dormancy.
Subject
Plant Science,Agronomy and Crop Science
Cited by
44 articles.
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