Abstract
An important goal of modern analyses of semen is to elucidate the molecular
traits of mammalian sperm chromatin structural abnormalities, defined here as
‘uncompensable’, that lead to abnormalities in fertility,
pronuclear formation, early embryo quality and pregnancy outcome. Sperm with
uncompensable nuclear abnormalities are able to fertilize oocytes both
in vivo and in vitro; however, due
to the uncompensable trait(s), the embryo development may be abnormal.
Uncompensable nuclear traits can be experimentally induced in bull sperm by a
mild thermal insult to the testis. Sperm nuclear morphology abnormalities seen
in ejaculates 11-days post stress are likely related to molecular changes in
chromatin observed 3-days post stress by the flow cytometric sperm chromatin
structure assay (SCSA). The SCSA measures the susceptibility of sperm nuclear
DNA to denaturation in situ. This susceptibility has
been correlated with the presence of DNA strand breaks that may be derived in
part by oxidative stress and possibly by a unique, abortive apoptotic
mechanism. The extent of DNA denaturation is not significantly related to the
level of disulfide bonding between the chromatin protamines. The use of human
sperm with uncompensable nuclear traits for artificial reproductive techniques
is also discussed. The goal of this research is to remove from semen doses
those sperm with uncompensable nuclear traits and thereby increase male
fertility potential.
Extra key words: male fertility potential, sperm
chromatin structure assay (SCSA).
Subject
Developmental Biology,Endocrinology,Genetics,Molecular Biology,Animal Science and Zoology,Reproductive Medicine,Biotechnology
Cited by
94 articles.
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