Dimethylsulfoxide and conjugated linoleic acids affect bovine embryo development in vitro

Abstract

Conjugated linoleic acids (CLA) are employed to overcome the bovine periparturitional negative energy balance. Especially of interest are trans10,cis12 -linoleic acid (t10c12-CLA) and cis9,trans11-linoleic acid (c9t11-CLA). Their impact on embryonic development, though, is not clear. Here, effects of both above-mentioned CLA on bovine in vitro-produced embryos were assessed. Zygotes (n = 2098) were allocated to one of seven groups: cultured with 50 or 100 µM of either c9t11-CLA or t10c12-CLA, with 14 or 28 mM DMSO or without supplement (control). Messenger RNA analysis of target gene transcripts (IGF1R, IGFBP2, IGFBP4, CPT2, ACAA1, ACAA2, FASN, SCD) via RT-qPCR was performed in single blastocysts. Cleavage rates did not differ, whereas development rates were decreased in both t10c12-supplemented groups in comparison to the unsupplemented group (31.7% ± 2.2 control vs 20.2% ± 2.0 50 µM t10c12 vs 21.0% ± 2.8 100 µM t10c12). Compared with the unsupplemented group, SCD was expressed at a lower level in embryos cultured with 50 µM c9t11-CLA. The relative amount of several transcripts was increased in embryos cultured with 14 mM DMSO in comparison to those that developed in the presence of 50 µM t10c12-CLA (IGFBP2, ACAA1, CPT2, FASN, SCD) or 50 µM c9t11-CLA (IGF1R, IGFBP2, ACAA1, CPT2, FASN, SCD). The molecular analyses show that CLA influence embryonic fat metabolism.