Author:
Astarini I. A.,Yan G.,Plummer J. A.
Abstract
Interspecific hybridisation within the genus Boronia was
attempted by using species with a range of chromosome numbers. Crosses were
possible between Western Australian species including those with
B. heterophylla as the female parent and pollen from
B. molloyae (n = 8),
B. megastigma (n = 7),
B. purdienana (n = 9) and
B. ramosa (n = 18).
Cytological studies revealed that B. heterophylla
genotypes had different chromosome numbers; ‘Red’ had
2n = 15, whereas ‘Moonglow’ and
‘Cameo’ had 2n = 14. The F
1 hybrid from B. heterophylla
‘Red’ × B. megastigma had
2n = 15. Pollen viability, examined by using
fluorescein diacetate, varied from 20% in
B. crenulata to 80% in
B. purdieana. Pollen of most species could be stored for
12 months in sealed containers at −20°C. Drying with silica gel
desiccant did not improve storage, nor did ultra-low temperature
(−196°C). Stored pollen was successfully used for interspecific
hybridisation.
Subject
Plant Science,Ecology, Evolution, Behavior and Systematics
Cited by
11 articles.
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