Effect of a probiotic (Lactobacillus sp.), yeast (Saccharomyces cerevisiae) and mycotoxin detoxifier alone or in combination on performance, immune response and serum biochemical parameters in broilers fed deoxynivalenol-contaminated diets

Abstract

Context Deoxynivalenol (DON) contamination of feedstuffs causes detrimental effects on animals and poultry. Dietary inclusion of microbial feed additives, such as probiotics and/or yeast, seems to be a useful approach for DON detoxification and reducing the toxin absorption from the gut. Aims This study was conducted to evaluate the synergetic effects of a probiotic (Lactobacillus spp.), yeast (Saccharomyces cerevisiae) and mycotoxin detoxifier on performance, serum chemical parameters and immune status of broiler chickens fed a DON-contaminated diet. Methods A total of 200 1-day-old female broilers (Hubbard®) were allocated to five dietary treatments with four replicates each in a completely randomised design. Experimental diets consisted of: (1) control diet (basal diet), (2) DON diet (basal diet contaminated with 10 mg/kg DON), (3) DON diet supplemented with 0.25% mycotoxin detoxifier (Mycofix® Plus), (4) DON diet supplemented with a combination of 0.4 g/kg probiotic and 1.5 g/kg yeast, and (5) DON diet supplemented with a combination of a probiotic, yeast and mycotoxin detoxifier. Key results The results showed that the DON diet significantly increased the feed conversion ratio compared with mycotoxin detoxifier and control diets. Administration of 0.4 g/kg probiotic along with 1.5 g/kg yeast to a DON-contaminated diet caused a significant enhancement of dressing percentage, leg relative weight and aspartate aminotransferase enzyme activity. The DON-contaminated diet reduced total protein, albumin, triglyceride and cholesterol concentrations, but consumption of a probiotic, yeast and mycotoxin detoxifier improved the levels of these parameters. The DON-treated birds showed a reduction in haematocrit, haemoglobin, red blood cells and blood lymphocyte percentage in comparison with control levels. The inclusion of a combination of a probiotic and yeast along with mycotoxin detoxifier diminished the increased heterophil: lymphocyte ratio by DON administration. The antibody titre against Newcastle disease virus vaccine increased by inclusion of a probiotic, yeast and Mycofix. Conclusions In summary, a combination of a probiotic and yeast along with mycotoxin detoxifier can improve the enzyme activity, immunity and haematological attributes, which are useful for DON-contaminated diet detoxification. Implications Dietary supplementation with a probiotic, yeast and mycotoxin detoxifier could be used as an alternative to detoxification DON in broiler chickens.