Detergent-resistant microdomains (lipid rafts) in endomembranes of the wild halophytes

Abstract

In the present work, we studied detergent-resistant membrane microdomains (DRM) of chloroplasts and mitochondria – organelles that provide photosynthesis and respiration in a plant cell. The objects of the study were euhalophyte Salicorniaperennans Willd., which relates to salt-accumulating plants and glycohalophyte Artemisia santonica L., which relates to salt-excluder plants. To get DRM, the chloroplast and mitochondria fractions were solubilised with a solution containing Triton X-100. The resulting material was introduced in sucrose gradient of 35–25–15–5% and centrifuged at 200000 g, 2 h. The presence of an opalescent detergent-resistant zone of membranes in 15% sucrose layer and a specific lipid composition of this zone were the signs of successful rafts obtaining of. The isolated DRM are sterol- and cerebroside-enriched (27–89% of the sum of membrane lipids) domains with a high degree of saturation of fatty acids composition (more than 50% of the sum). The main DRM-specific lipids of chloroplast of A. santonica glycohalophyte are cerebrosides, whereas those of S. perennans euhalophyte are sterols. The revealed differences in the composition of raft-forming lipids in chloroplast and mitochondria halophyte membranes, differing in the salt-resistance strategy, suggest the participation of rafts in salt-resistance mechanisms.