Effects of wild-type, AR1 and AR37 endophyte-infected perennial ryegrass on dairy production in Victoria, Australia

Abstract

A 3-year experiment compared the effects of wild-type ryegrass endophyte and two novel endophytes on milk production in dairy cattle. On three 9.9-ha farmlets in West Gippsland, Victoria, pasture swards were established that were dominant in perennial ryegrass (Lolium perenne) infected with either wild-type, AR1 or AR37 endophytes. Each farmlet was stocked with 25 spring-calving Holstein–Friesian cows, which rotationally grazed nine paddocks within their farmlet during three lactations over 3 years. The three endophytes are known to produce different alkaloids, with wild-type producing ergot alkaloids, lolitrems and peramine, AR1 producing peramine and AR37 producing epoxy-janthitrems. These alkaloids were present in fresh pasture as well as hay and silage made from that pasture. Grazed pasture comprised 53% of estimated annual DM intake. The proportion was least from December to March when the daily ration of 2 kg DM/cow.day cereal grain was increased to 6 kg/day and forage supplements were fed consisting of purchased lucerne (Medicago sativa) hay and pasture silage grown on the farmlets. There were no differences in pasture accumulation rates or nutritive characteristics of ryegrass pastures on the three farmlets and no differences in the production of milk, fat or protein by cows grazing pasture infected with the three endophytes. Ryegrass staggers was only observed in four cows consuming the wild-type-infected ryegrass in the first year when the highest concentrations of lolitrem B were recorded in pasture. Soil samples showed lower numbers of root aphids (Aploneura lentisci), mealybugs (Pseudococcidae) and pasture tunnel moths (Philobota spp.) beneath ryegrass infected with the AR37 endophyte compared with the other two endophytes. Numbers of redheaded (Adoryphorus couloni) and blackheaded (Aphodius tasmaniae) cockchafers did not differ between treatments. Under dairy management and supplementary feeding regimes common to south-eastern Australia, the novel endophytes AR1 and AR37 had no effect on the milk production compared with the wild-type endophyte, and did not cause ryegrass staggers.