Effects of cobalt/vitamin B12 status in ewes on ovum development and lamb viability at birth

Abstract

Scottish Blackface ewes from cobalt-deficient farmland were fed a diet containing 0.06 mg cobalt per kg dry matter from approximately 30 days before embryo recovery/transfer until lambing. Ewes remained untreated (–Co; n = 82) or were given an intraruminal cobalt-containing bolus to compensate for the dietary deficit (+Co; n = 82). Ewes used as embryo donors (–Co, n = 17; +Co, n = 16) were artificially inseminated with semen from a single Suffolk sire. Day 6 embryos obtained from –Co and +Co donors were transferred in singleton to –Co and +Co recipients in a 2 × 2 factorial-designed experiment to determine the effects of cobalt/vitamin B12 status during the periconception period (factor 1) and pregnancy (factor 2) on lamb viability at birth. Mean (± s.e.m.) circulating concentrations of vitamin B12 in –Co and +Co donors at ovum recovery were 182 ± 10 and 1288 ± 64 pmol L–1, respectively (P < 0.001), and the number of corpora lutea per ewe ovulating was 9.9 ± 1.6 and 14.4 ± 1.3, respectively (P < 0.05). Treatment did not affect the proportion of recovered ova that contained >32 cells (viable) or the median stage of development (late morula), but viable ova recovered from –Co v. +Co ewes had a better morphological grade (2.0 ± 0.1 v. 2.20 ± 0.04, respectively; P < 0.01). There was no effect of treatment on the proportion of recipient ewes that became pregnant. Circulating concentrations of vitamin B12 were lower in –Co than +Co ewes during pregnancy (P < 0.001) and at birth in lambs born to –Co ewes compared with those born to +Co ewes (P < 0.001). There was no effect of donor or recipient cobalt/vitamin B12 status on lamb birthweight, neonatal vigour or neonatal rectal temperatures, but lambs derived from +Co v. –Co embryo donors were more active in the first 3 days after birth (P < 0.05). Results show that sub-clinical cobalt/vitamin B12 deficiency reduces ovulatory response in superovulated ewes and that periconception nutrition can affect neonatal lamb behaviour.