Perkembangan Aplikasi Teknik Kriopreservasi untuk Konservasi dan Mendukung Program Pemuliaan Tanaman

Abstract

<p>In the beginning, cryopreservation<br />technique was primarily used for germplasms<br />long-term storage as passive collection because cell division<br />and metabolism process can be stopped at super low<br />temperature, commonly in liquid nitrogen. The technique is<br />suitable for vegetative propagated plants and recalcitrant<br />seeds. Recently, its application is extending for storing many<br />species and orthodox seeds. In this paper, the development<br />of cryopreservation application is discussed. In Indonesia,<br />cryopreservation is being studied but the application of<br />cryopreservation has been significantly developed abroad.<br />The application of cryopreservation technique is not only for<br />preserving passive collections but also for storing active<br />collections, including to provide plant materials for hybridization,<br />cellular engineering, genetic transformation, as well<br />as pathogens eradication or cryotherapy. It is concluded that<br />cryopreservation plays an important role in conventional and<br />modern plant breeding program.</p>