The ameliorative effect of Aspergillus awamori on aflatoxin B1-induced hepatic damage in rabbits

Author:

Abdelhady D.H.1,El-Abasy M.A.2,Abou-Asa S.S.E.3,Elbialy Z.I.4,Shukry M.5,Hussein A.H.6,Saleh A.A.7,El-Magd M.A.8

Affiliation:

1. Department of Clinical Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, 33516 Kafrelsheikh, Egypt.

2. Department of Poultry and Rabbit Diseases, Faculty of Veterinary Medicine, Kafrelsheikh University, 33516 Kafrelsheikh, Egypt.

3. Department of Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, 33516 Kafrelsheikh, Egypt.

4. Department of Fish Processing and Biotechnology, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, 33516 Kafrelsheikh, Egypt.

5. Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, 33516 Kafrelsheikh, Egypt.

6. Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, 0045 Zagazig, Egypt.

7. Department of Animal Wealth Development, Veterinary Genetics & Genetic Engineering, Faculty of Veterinary Medicine, Zagazig University, 0045 Zagazig, Egypt.

8. Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, 33516 Kafrelsheikh, Egypt.

Abstract

This study was conducted to investigate the effect of dietary supplementation of Aspergillus awamori on aflatoxin B1 (AFB1)-induced liver damage in rabbits. Administration of AFB1 (0.3 mg/kg diet) led to a significant reduction in body weight, body weight gain, total feed intake, total serum proteins, albumin, high density lipoprotein-cholesterol, phagocytic activity, phagocytic index, and the antioxidant enzyme, glutathione peroxidase (GPx). Moreover, AFB1 administration was associated with a significant increase in feed conversion ratio, lipid peroxidation and serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma-glutamyl transferase, lactate dehydrogenase and total bilirubin. In addition, livers of AFB1-supplemented animals showed fatty degeneration with vacuolisation, focal areas of necrosis, mononuclear cells infiltration hyperplasia of bile ducts and sinusoids. A significant increase in the hepatic expression of the biotransformation gene (Cyp3A6), stress-sensitive genes (HO1 and SOD1), and inflammation-related genes (IL6, TNFa, NF-kB, and Cox2) was also observed. Supplementation of the diets with 0.05, 0.1 or 0.15% A. awamori ameliorated all AFB1 deleterious effects with the best improvement observed at the lowest concentration. This is the first investigation to report that supplementation of rabbit diets with A. awamori has an ameliorative effect against AFB1-induced liver damage possibly through preventing hepatic oxidative stress, promoting the antioxidant defence systems, and inhibiting expression of Cyp3A6, HO1, SOD1, IL6, TNFa, NF-kB, and Cox2. Therefore, A. awamori could be used as a potential preventive or therapeutic agent for aflatoxicosis.

Publisher

Wageningen Academic Publishers

Subject

Public Health, Environmental and Occupational Health,Toxicology,Food Science

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