Digestive physiological characteristics of black soldier fly larvae reared on five organic wastes

Abstract

Black soldier fly larvae (BSFL) are able to utilise a broad range of organic wastes to fulfil their growth needs. To acquire this basic knowledge of its digestive adaptation to various food, five organic wastes (soybean meal [SBM], wheat bran [WB], beer yeast [BY], kitchen waste [KW] and chicken manure [CM]) were fed to 3-day-old BSFL for 16 days. The growth performance, luminal pH of the gut, midgut histology, digestive enzyme activity and intestinal bacterial microbiota of the larvae were assessed. Growth performance was greatest in the KW group followed by the SBM group and was worst in the CM group. Nutrient compositions of larvae were not significantly affected by those of the food sources, with the exception of crude ash. The ultrastructural observations of midgut showed the number of mitochondria in CM and BY groups was less than other three groups. Twenty-four hours after feeding, weakly acidic, acidic, strongly acidic, and alkaline luminal pH values were detected in the anterior, middle and posterior segments of the gut in all groups, but the luminal pH values of the hindgut varied with food source. Significant lipase and esterase activity, cellulase activity, and amylase activity were detected in the intestines of larvae reared on KW, WB and BY, respectively, revealing that digestive enzyme activity was closely associated with the nutrient composition of the food source. Bacterial composition and diversity differed significantly between groups and were characterised by specific indicator bacteria, which may play important roles in food digestibility. The results suggested that different food sources induced adaptive physical, chemical and biological changes in the digestive tracts of BSFL and may indicate that BSFL developed specific strategies for nutrient utilisation and accumulation. The knowledge acquired here will be beneficial for developing rearing protocols to optimise bioconversion in this insect for their various applications.