Mycotoxins content and its association with changing patterns of Fusarium pathogens in wheat in the Czech Republic

Abstract

Mycotoxin content in 244 samples of wheat ears randomly collected during 2014 and 2015 from various localities in the Czech Republic was analysed using liquid chromatography coupled to mass spectrometry (LC-MS). Mean mycotoxin concentration in 2014 was highest for deoxynivalenol (DON; 760 μg/kg), followed by zearalenone (ZEA; 115 μg/kg), 3-acetyldeoxynivalenol (3-ADON; 88 μg/kg), deoxynivalenol-3-glucoside (83 μg/kg), and enniatins (ENNs; 102 μg/kg). In 2015, DON (66 μg/kg) also had the highest concentration level, followed by ENNs (35 μg/kg), nivalenol (2 μg/kg), and beauvericin (2 μg/kg). The maximum limit for DON in the European Union (1,250 μg/kg) was exceeded in 2% of samples, and the maximum limit for ZEA (100 μg/kg) was exceeded in 0.8% of samples. Fusarium species causing head blight were identified using PCR assays. During 2014-2015, Fusarium poae considerably dominated (48.7% average value of occurrence in the samples). Other species were detected in much lower frequencies in both years: Fusarium graminearum (average frequency of occurrence 13.7%), Fusarium avenaceum (11.9%), Fusarium culmorum (4.2%), and Fusarium equiseti (2.9%). Fusarium langsethiae was identified only in 2015, at a frequency of 10.2%, and Fusarium sporotrichioides was present only sporadically in 2014.