Deoxynivalenol and nivalenol analysis in soybean and soy flour

Author:

Barros G.1,García D.1,Oviedo S.1,Ramirez M.1,Torres A.1,Chulze S.1

Affiliation:

1. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36 Km 601, Río Cuarto, Córdoba, Argentina

Abstract

A rapid and accurate method of quantifying deoxynivalenol (DON) and nivalenol (NIV) in soybean and soy flour is described. The samples were extracted with acetonitrile:water (84:16, v/v) and cleaned through a solidphase extraction (SPE) column. The mycotoxins were separated, detected and quantified by reversed-phase high performance liquid chromatography (HPLC) with UV detection (220 nm) using water:methanol (88:12, v/v) as mobile phase. Characteristics of this in-house method such as accuracy, precision and detection and quantification limits were defined by means of a recovery test with spiked soybean and soy flour samples. The detection limit (LOD) was 0.1 µg/g for DON and 0.2 µg/g for NIV, based on a signal-noise ratio 3:1. Quantification limit (LOQ) was established as three times the detection limit.

Publisher

Wageningen Academic Publishers

Subject

Public Health, Environmental and Occupational Health,Toxicology,Food Science

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