The effect of Clostridium tyrobutyricum Spo0A overexpression in the intestine of mice

Author:

Liang Q.1,Liu J.12,Wei J.1,Jia J.1,Shen H.1,Chen W.2,Liang W.2,Gao B.2,Xu Z.2,Zhang L.13

Affiliation:

1. Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China P.R.

2. Guangdong Provincial Key Laboratory of Livestock and Poultry Disease Control, Guangdong Provincial Institute of Veterinary Public Health, Public Health Laboratory, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong 510642, China P.R.

3. Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China P.R.

Abstract

Clostridium tyrobutyricum shows probiotic properties and can affect the composition of gut microbiota and regulate the intestinal immune system. Compared with other probiotics, this spore-producing bacterium shows unparalleled advantages in commercial production. In addition to being resistant to extreme living environments for extended periods, its endophytic spores are implicated in inhibiting cancer cell growth. We speculated that C. tyrobutyricum spores can also promote gut health, which mean it can maintain intestinal homeostasis. To date, the beneficial effects of C. tyrobutyricum spores on gut health have not been reported. In this study, a Spo0A-overexpressing C. tyrobutyricum strain was developed to increase spore production, and its probiotic effects on the gut were assessed. Compared with the wild-type, the engineered strain showed significantly increased sporulation rates. Mice administered with the engineered strain exhibited enhanced intestinal villi and the villus height/crypt depth ratio, weight gain and improved Firmicutes/Bacteroidetes ratio to facilitate intestinal homeostasis. This study demonstrated for the first time that enhanced spore production in C. tyrobutyricum can improve intestinal homeostasis, which is advantageous for its commercial application in food and pharmaceutical industry.

Publisher

Wageningen Academic Publishers

Subject

Microbiology (medical),Microbiology

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