Comparative study of water and temperature relations of growth and T-2/HT-2 toxin production by strains of Fusarium sporotrichioides and Fusarium langsethiae

Abstract

This study has compared the effect of water activity (aw, 0.995-0.93) and two temperatures (15, 25 °C) on the growth and HT-2 and T-2 toxin production by three strains of Fusarium sporotrichioides and Fusarium langsethiae in an oat-based medium. There were very little intra-strain differences for each of the species in relation to growth at 0.995-0.93 aw and 15 and 25 °C. Inter-species comparisons showed that at 0.995 and 0.98 aw growth was similar at both temperatures. However, under water stress at 0.95 and 0.93 awF. sporotrichioides strains were more tolerant than those of F. langsethiae. Statistical analyses showed that aw and temperature were significant factors for both species, while strain differences were not (P=0.05). In contrast, the patterns of production of T-2 and HT-2 toxins were very different from that for growth. For F. sporotrichioides, a generally higher amount of toxin was produced at 15 than at 25 °C, with a maximum produced at 0.995 aw, while the production decreased with water stress. Interestingly, maximum production of T-2+HT-2 toxin was achieved by F. langsethiae at 0.98 aw and 25 °C, with very little produced at 0.93 aw. Statistically, aw was the most significant (P=0.05) factor for the mean of the three strains of both species with a significant inter-species difference in terms of toxin production. This was confirmed by the changes in the ratio of HT-2:T-2 toxin in relation to the aw × temperature conditions studied. These results are discussed in the context of the effect of environment on the relative importance of these two species in contaminating oats with these toxins.