Inhibitory effect of Enterobacter cloacae 3J1EC on Aspergillus flavus 3.4408 growth and aflatoxin production

Abstract

Aspergillus flavus can easily infect major agricultural products and produce aflatoxin. In this study, we investigated the effect of the biocontrol bacterium Enterobacter cloacae 3J1EC on the growth of A. flavus strain 3.4408. The biocontrol bacterium played a key role in preventing infection by A. flavus. E. cloacae 3J1EC was found to inhibit the growth of A. flavus 3.4408 mycelial pellets and reduce the production of aflatoxin by 96.9%. We found differential expression between the control and the treatment groups in the transcriptome of A. flavus 3.4408. Gene ontology (GO) analysis indicated that E. cloacae 3J1EC induced the down-regulated expression of cellular component and molecular function, while its effects on the up-regulated expression indicated the relationship of biological process and molecular function. Thus, these results suggest that E. cloacae 3J1EC decreased aflatoxin production via down-regulated gene expression in terms of aflatoxin biosynthesis. In summary, E. cloacae 3J1EC can be employed as an alternative for the biological control of A. flavus 3.4408.