Cytotoxicity study of deoxynivalenol on human embryo liver and hepatoma cell

Abstract

To investigate the cytotoxicity of deoxynivalenol (DON) on human embryo liver CCC-HEL-1 and hepatoma cell line HepG2 cell models, both cell experience and metabolomic approach were studied. For the cell evaluation, cells viabilities of CCC-HEL-1 and HepG2 were decreased in both a time- and dose-dependent manner at concentration range from 0.08~10 μmol/l, after which the concentration of 1 μmol/l DON was selected for the next experiments. A higher production of reactive oxygen species (ROS) in DON treated CCC-HEL-1 cells was found after 2 h treatment compared with the HepG2 group, while ROS generation was significantly dropped after 48 h in both models. DON-treated CCC-HEL-1 and HepG2 cells displayed significantly decreased percentages of ΔΨm loss. For the metabolomic study based on liquid chromatography quadrupole time-of-flight mass spectrometry, it was notable that certain amino acids identified in the two DON-treated groups were upregulated. The pathway analysis also revealed that amino acid metabolism played a crucial role underlying DON exposure in the two studied models. Our results provided metabolic evidence that further confirmed the toxicological potential of DON to disturb amino acid and lipid metabolism in human embryo liver cells.