The effect of repeated barrel racing on blood biomarkers and physiological parameters in Quarter Horses

Abstract

The objective of this study was to evaluate barrel horses undergoing a field test with one repetition. Quarter-Horses were used (14 males and females; average age: 6.5 years old), and they ran two times for 5 min in the same field. Six different periods were evaluated: rest (T-0), immediately after the first (T-1) and second races (T-2), and after 15 (T-15), 30 (T-30) and 240 min (T-240) of recovery. Heart rate (HR), speed, distance and duration were measured using a HR monitor with GPS during the races. Blood was collected to determinate glucose, lactate, total proteins (TP), albumin, globulin, urea, creatinine, uric acid (UA), triglycerides, cholesterol, non-esterified fatty acids (NEFA), gammaglutamyltransferase (GGT), creatine kinase (CK), and cortisol concentrations, as well as to perform a haemogram. The results were analysed using ANOVA and Tukey’s tests using a P-value of 5%, and they showed a maximum HR>200 bpm after both races. The largest %HR>>170 bpm occurred in the second race (P<0.05). Glucose and lactate concentrations were elevated at T-2 (P<0.05). TP, creatinine, UA and triglycerides concentrations remained elevated at T-1, T-2 and T-30 (P<0.05). TP and creatinine concentrations returned to basal concentrations at T-30 (P>0.05), and UA and triglycerides returned to base concentrations at T-240 (P>0.05). NEFA, cholesterol, GGT and CK concentrations did not change (P>0.05). Cortisol concentrations were higher at T-2 (P<0.05) but at T-240 were similar to T-0 (P>0.05). Red blood cell concentrations and haematocrits had higher values after the second race (P<0.05). These results showed that barrel horses had intense exercise and that two races with a short rest interval between them produced similar metabolic and physiologic adaptations between races, showing that the proposed protocol may be a useful tool for field characterisation of horses’ training and may contribute to the implementation of adequate training and nutritional programs.