Selenium nanoparticles synthesized via green methods from Calluna vulgaris extract: Exploring their antioxidant and antibacterial activities
Author:
Erdem Ecem1ORCID, Aydın Acar Çiğdem1ORCID
Affiliation:
1. BURDUR MEHMET AKİF ERSOY UNIVERSITY
Abstract
This study introduces a sustainable and environmentally friendly method for synthesizing selenium nanoparticles (SeNPs) by using Calluna vulgaris as a reducing agent. The process involves the addition of Na2SeO3 to a C. vulgaris aqueous solution, followed by reduction with ascorbic acid. UV-Vis spectroscopy confirmed SeNP formation, with a distinct absorption peak at 289 nm. Morphological analysis via Scanning Electron Microscopy (SEM) revealed spherical nanoparticles below 100 nm, as corroborated by Transmission Electron Microscopy (TEM) images displaying sizes ranging from 42.91 to 66.93 nm. Energy Dispersive Spectroscopy (EDS) confirmed the presence of selenium. Antibacterial assessments demonstrated the efficacy of C.vulgaris Selenium Nanoparticles (Cv-SeNPs) against gram-positive (Enterococcus faecalis, Staphylococcus aureus) and gram-negative bacteria (Escherichia coli). Cv-SeNPs exhibited notable antibacterial activity, particularly against E. Faecalis. In terms of antioxidant activities, Cv-SeNPs exhibited significant scavenging potential against DPPH and ABTS radicals, with low IC50 values of 24.72 and 16.87 µg/mL, respectively. The scavenging activities increased with concentration, reaching 86.6% for DPPH and 99.7% for ABTS at specific concentrations. The inclusion of ascorbic acid as a capping agent further augmented the free radical scavenging capabilities, indicating a synergistic relationship between selenium nanoparticles and capping agents. This research underscores the dual functionality of Cv-SeNPs as effective antibacterial agents and potent antioxidants. The green synthesis methodology utilizing C. vulgaris offers a sustainable approach for producing selenium nanoparticles with desirable characteristics, suggesting potential applications in medicine and industry. Further research on biomedical and industrial uses of Cv-SeNPs is needed.
Publisher
International Journal of Secondary Metabolite
Reference28 articles.
1. Adibian, F., Ghaderi, R.S., Sabouri, Z., Davoodi, J., Kazemi, M., Ghazvini, K., Youssefi, M., Soleimanpour, S., & Darroudi, M. (2022). Green synthesis of selenium nanoparticles using Rosmarinus officinalis and investigated their antimicrobial activity. BioMetals, 35, 147–158. https://doi.org/10.1007/s10534-021-00356-3 2. Barzegarparay, F., Najafzadehvarzi, H., Pourbagher, R., Parsian, H., Ghoreishi, S.M., & Mortazavi-Derazkola, S. (2023). Green synthesis of novel selenium nanoparticles using Crataegus monogyna extract (SeNPs@ CM) and investigation of its toxicity, antioxidant capacity, and anticancer activity against MCF-7 as a breast cancer cell line. Biomass Conversion and Biorefinery, 1-10. https://doi.org/10.1007/s13399-023-04604-z 3. Cittrarasu, V., Kaliannan, D., Dharman, K., Maluventhen, V., Easwaran, M., Liu, W.C., Balasubramanian, B., & Arumugam, M. (2021). Green synthesis of selenium nanoparticles mediated from Ceropegia bulbosa Roxb extract and its cytotoxicity, antimicrobial, mosquitocidal and photocatalytic activities. Scientific reports, 11(1), 1032. https://doi.org/10.1038/s41598-020-80327-9 4. Gangadoo, S., Stanley, D., Hughes, R.J., Moore, R.J., & Chapman, J. (2017). The synthesis and characterisation of highly stable and reproducible selenium nanoparticles. Inorganic and Nano Metal Chemistry, 47(11), 1568 1576. https://doi.org/10.1080/24701556.2017.1357611 5. Ge, Y.M., Xue, Y., Zhao, X.F., Liu, J.Z., Xing, W.C., Hu, S.W., & Gao, H.M. (2024). Antibacterial and antioxidant activities of a novel biosynthesized selenium nanoparticles using Rosa roxburghii extract and chitosan: Preparation, characterization, properties, and mechanisms. International Journal of Biological Macromolecules, 254, 127971. https://doi.org/10.1016/j.ijbiomac.2023.127971
|
|