Analysis of phytochemical composition and biological activities of Helichrysum pallasii (Sprengel) ledeb leaves
Author:
IŞIK Nejmi1ORCID, MÜKEMRE Muzaffer2ORCID, TÜRKER Rabia Sena1ORCID, ZENGİN Gokhan3ORCID, DALAR Abdullah4ORCID
Affiliation:
1. VAN YUZUNCU YIL UNIVERSITY 2. HAKKARI UNIVERSITY 3. SELCUK UNIVERSITY 4. VAN YUZUNCU YIL UNIVERSITY, FACULTY OF PHARMACY, PHARMACY PR.
Abstract
This study aimed at the investigation of in vitro biological activities (antioxidant and enzyme inhibition) and phytochemical composition of various extracts (ethanol maceration, aqueous infusion, and aqueous decoction) obtained from leaf organs of Helichrysum pallasii, which has been traditionally used as herbal tea and medicine in Eastern Anatolia.
Experimental analysis showed that ethanol-based extract had higher total phenolic content (TPC: 103 mg GAE/gE) and showed superior antioxidant potentials (FRAP: 2205 μmol Fe2+/gE; ORAC: 2540 μmol Trolox Eq./gE; DPPH: IC50=0.58 mg/ml; CUPRAC: IC50=0.37 mg/ml; Phosphomolybdenum: IC50=1.34 mg/ml ve metal chelation: IC50=1.42 mg/ml) and enzyme inhibition (Acetylcholinesterase: IC50=1.49 mg/ml; Butyrylcholinesterase: IC50=1.98 mg/ml; Tyrosinase: IC50=0.68 mg/ml; Alpha-amylase: IC50=2.09 mg/ml; Alpha-glucosidase: IC50=0.51 mg/ml; and Pancreatic lipase: IC50=42.5 μg/ml) and contained higher amounts of phenolic (chlorogenic acid isomers and rutin) and fatty acid (palmitic, linoleic, and linolenic acids) compounds than traditional preparations (infusion and decoction).
The current study's findings indicate that the leaves of the Helichrysum pallasii are a source of phytochemicals with strong antioxidant and enzyme inhibitory properties, implying that it could be a candidate for biotherapeutic agent research and development.
Publisher
International Journal of Secondary Metabolite
Subject
Plant Science,Biochemistry,Biophysics,Biotechnology
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