Affiliation:
1. PAMUKKALE UNIVERSITY, INSTITUTE OF SCIENCE
2. Pamukkale University, Faculty of Science
3. Pamukkale Üniversitesi, Uygulamalı Bilimler Fakültesi, Organik Tarım İşletmeciliği Bölümü
Abstract
The aim of this study is to investigate the antifungal activity of propolis against V. dahliae Kleb. under both in vitro and in vivo conditions. Firstly, the inhibitory effect of the propolis on mycelial growth in Potato Dextrose Agar (PDA) media containing its ethanol extract (PE) at various concentrations (0.003, 0.06, 0.125, 0.25, 0.5, and 1 ppm/mL) was investigated under in vitro conditions. Then to assess the effect of PE on non-defoliating (PHCVd3 isolate) and defoliating (PHCVd47 isolate) pathotypes of V. dahliae, the varieties Giza 45 (resistant), Carmen (tolerant), and Acala SJ2 (susceptible) treated with PEE (1 ppm/mL) were observed in the plant growth chamber up to the 4-6 leaf stage. The whole in vitro experiments were carried out with three replicates, and the studies in vivo experiment were with five replicates depending on a completely randomized parcels design. The most effective dose of PEE with1 ppm/mL dose resulted in 75.2% suppression against the PHCVd3 isolate, while the effect of the same dose against the PHCVd47 isolate was 74.4%. The lowest disease severity index (DSI) values against PHCVd3 and PHCVd47 isolates in cotton cultivars treated with PE were 1.34 and 1.64 in the Giza 45, respectively, and the highest DSI values were 3.80 and 3.90 in the Acala SJ2 cultivar in vivo experiment, respectively. The findings indicate that PE treatment has a promising effect against cotton wilt disease that could be combined with known plant protection strategies.
Funder
The Scientific Research Coordination Unit of Pamukkale University of Turkey
Publisher
International Journal of Secondary Metabolite
Subject
Plant Science,Biochemistry,Biophysics,Biotechnology
Reference48 articles.
1. Abd-El-Kareem, F., Saied, N.M., Abd-Elgawad, M.M.M. (2017). Postharvest application with propolis for controlling white rot disease of green bean pods. Agricultural Engineering International: CIGR Journal, 310-314. http://orcid.org/0000-0002-4731-5988
2. Abo-Elyousr, K.A.M., Al-Qurashi, A.D., Almasoudi, N.M. (2021). Evaluation of the synergy between Schwanniomyces vanrijiae and propolis in the control of Penicillium digitatum on lemons. Egyptian Journal of Biological Pest Control, 31, 66. https://doi.org/10.1186/s41938-021-00415-4
3. Agrios, G.N. (2005). Plant Pathology. Academic Press (5th ed.), New York.
4. Ali, A.M., Kunugi H. (2020). Apitherapy for age-related skeletal muscle dysfunction (sarcopenia): A review on the effects of royal jelly, propolis, and bee pollen. Foods, 9(10), 1362. https://doi.org/10.3390/foods9101362
5. Araújo, M.J.A.M., Bosco, S.D.M.G., Sforcin, J.M. (2016). Pythium insidiosum: inhibitory effects of propolis and geopropolis on hyphal growth. Brazilian Journal of Microbiology, 47, 863-869. https://doi.org/10.1016/j.phymed.2019.153098
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