Bioactive compounds of Arctostaphylos uva-ursi wild-growing populations from Bulgaria
Author:
NİKOLOVA Milena1ORCID, ANEVA Ina1ORCID, ZHELEV Petar2ORCID, BERKOV Strahil1ORCID, YANKOVA-TSVETKOVA Elina1ORCID
Affiliation:
1. Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences 2. University of Forestry
Abstract
Arctostaphylos uva-ursi (L.) Sprengel (bearberry), Ericaceae is a valuable medicinal plant with diuretic and uroantiseptic action which is mainly due to arbutin. In Bulgaria the species is considered as rare. Content assessment of bioactive compounds of A. uva-ursi leaves from three natural populations from Bulgaria was the aim of the present study. Leaf samples were collected from Pirin, Vitosha, and Rhodope Mountains. Bioactive compounds in the methanolic extracts of the samples were analysed by GC/MS. Total phenolic content was determined using Folin–Ciocalteu reagent. Arbutin, quinic acid and gallic acid were detected in the highest amounts. Catechine, 4-hydroxybenzoic acid, chlorogenic acid, triterpenes (α- and β-amyrin, uvaol and lupeol) and other primary and secondary metabolites were found, also. Differences in the content of individual compounds between samples of different origin were established. The highest total phenolic (182.98 mg GAE g-1) and arbutin (8.4%) content was found in the sample from Vitosha Mountain. The presented data characterizes the profile of bioactive compounds in the Bulgarian bearberry raw material for the first time.
Funder
Bulgarian National Science Fund, Bulgarian Ministry of Education and Science
Publisher
International Journal of Secondary Metabolite
Reference18 articles.
1. Asensio, E., Vitales, D., Pérez, I., Peralba, L., Viruel, J., Montaner, C., Vallès, J., Garnatje, T., & Sales, E. (2020). Phenolic Compounds Content and Genetic Diversity at Population Level across the Natural Distribution Range of Bearberry (Arctostaphylos uva-ursi, Ericaceae) in the Iberian Peninsula. Plants, 9(9), 1250. https://doi.org/10.3390/plants9091250 2. Bhalla, M., R. Mittal, M., Kumar, A., & Kushwah S. (2023). Pharmacological Aspects of a Bioactive Compound Arbutin: A Comprehensive Review. Biointerface Research in Applied Chemistry, 13(2), 119. https://doi.org/10.33263/BRIAC132.119 3. Berkov, S., Pechlivanova, D., Denev, R., Nikolova, M., Georgieva, L., Sidjimova, B., Bakalov, D., Tafradjiiska, R., Stoynev, A., Momekov, G., & Bastida J. (2021). GC-MS analysis of Amaryllidaceae and Sceletium-type alkaloids in bioactive fractions from Narcissus cv. Hawera. Rapid Communications in Mass Spectrometry, 35(14), e9116. https://doi.org/10.1002/rcm.9116 4. Boo, Y.C. (2021). Arbutin as a Skin Depigmenting Agent with Antimelanogenic and Antioxidant Properties. Antioxidants, 10(7), 1129. https://doi.org/10.3390/antiox10071129 5. de Arriba, S.G,, Naser, B., & Nolte, K.U. (2013). Risk assessment of free hydroquinone derived from Arctostaphylos uva-ursi folium herbal preparations. International Journal of Toxicology, 32(6), 442-53. https://doi.org/10.1177/1091581813507721.
|
|