The Effects of Rod and Round-Like Nanohydroxyapatites on Allium cepa Root Meristem Cells
Author:
GÜNEŞ Merve1ORCID, YALCİN Burcin1ORCID, KURŞUN Ayşen Yağmur1ORCID, TAGORTİ Ghada1ORCID, YAVUZ Emre2ORCID, AKARSU Esin3ORCID, KAYA Nuray1ORCID, KAYA Bülent1ORCID
Affiliation:
1. Akdeniz University, Faculty of Sciences, Department of Biology 2. Akdeniz University, Faculty of Science, Department of Chemistry 3. Akdeniz University, Faculty of Sciences, Department of Chemistry,
Abstract
Biomaterials are engineered products that are widely used in many areas of medicine fields such as orthopaedic applications, facial and maxillofacial surgery, artificial heart parts, metal parts, and implantable devices. These materials are widely used in medicine because they are biocompatible with the organism, non-allergic, and are resistant to physical and chemical factors. Hydroxyapatites are bioactive calcium phosphate ceramics that are compatible with tissues. Nano-sized hydroxyapatite has been produced to increase their bioactivity. Although there are advantages to the use of nanoparticles in medicine and therapy, the potential toxicity of these compounds on the ecosystem and human health are of concern. One of the key issues to be investigated is whether the different forms of the same nanoparticle will cause differences in genotoxicity. Herein, the potential genotoxic effects of rod and round forms of nano-sized hydroxyapatites (nHAs) were evaluated using the Allium cepa Single Cell Gel Electrophoresis (SCGE) method. Results had shown that the round form of nHA in the A. cepa meristem root tip cells caused statistically significant genotoxicity at 25 µg/mL concentration in terms of tail intensity and tail moment. This study indicated small-sized-nanohydroxyapatite-induced genotoxicity and cell death in A. cepa. This study has shown that the physical properties of nanoparticles affect potential toxicity mechanisms.
Funder
Scientific Research Projects Coordination Unit of Akdeniz University
Publisher
Gazi University Journal of Science
Subject
Multidisciplinary,General Engineering
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