The Study of Interaction Activity of Nickel (ll) Phthalocyanine Complex Bearing Tetra Substituted Phenoxy-3-Methoxybenzoic Acid Groups with DNA

Author:

ARSLANTAS Ali1,AĞIRTAŞ Mehmet Salih2

Affiliation:

1. IZMIR BAKIRCAY UNIVERSITY

2. VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ

Abstract

Nickel phthalocyanine complex containing 4-(3,4-dicyanophenoxy)-3-methoxybenzoic acid group was synthesized and specified by way of FT-IR, NMR, UV/Vis procedures. The binding of PcNi complex to CT-DNA was examined via electronic absorption titration, emission titration, melting temperature, viscosity measurement, and agarose gel electrophoresis technics, respectively. The DNA interaction activity of PcNi against CT-DNA was studied by way of UV/Vi titrations, fluorescence spectra, farther by conducting melting point, viscosity procedures in the buffer of a pH 7.02. The obtained outcomes from these methods demonstrated that PcNi indicated substantial binding affinity to the DNA via intercalating by the binding constant of 1.31 x 106 m-1. Further, the interaction activity of the complex on CT-DNA was investigated by which the electrophoresis technique and this procedure indicated that PcNi complex exhibits strong binding affinity on the DNA.

Publisher

Gazi University Journal of Science

Subject

Multidisciplinary,General Engineering

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