Development of Rapid Screening of Campylobacter spp. and the GyrA Mutants Using PCR-DNA Strip and Real-Time PCR

Author:

KOJIMA Chiaki1,KISHIMOTO Michiru2,MIYATA Machiko1,NOMURA Sayumi1,EZAKI Takayuki1

Affiliation:

1. Department of Microbiology, Gifu University Graduate School of Medicine

2. Department of Nutritional Sciences, Nagoya University of Arts and Sciences

Publisher

Japanese Society of Food Microbiology

Subject

General Medicine

Reference21 articles.

1. 1) Aiba, M., Chuma, T. and Okamoto, K.: Relationship of emergence of antimicrobial resistance of Campylobacter and Salmonella spp. on broiler farms. J. Jpn. Vet. Med. Assoc., 65, 147–152 (2012).

2. 2) Antti, H., Jari, J., Pirkko, K., Hannele, J. S., Anja, S. and Pentti, H.: GyrA polymorphism in Campylobacter jejuni: detection of gyrA mutations in 162 C. jejuni isolates by single-strand conformation polymorphism and DNA sequencing. Antimicrob. Agents Chemother., 46, 2644–2647 (2002).

3. 3) Chuma, T., Maeda, T., Niwa, H. and Okamoto, K.: Acquisition of quinolone resistance and point mutation of the gyrA gene in Campylobacter jejuni isolated from broilers and in vitro-induced resistant strains. Public Health, 66, 155–160 (2003).

4. 4) Clinical and Laboratory Standards Institute: Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria. CLSI document M45-A2 (2010).

5. 5) Engberg, J., Aarestrup, F. M., Tayler, D. E., Gerner-Smidt, P. and Nachamkin, I.: Quinolone and macrolide resistance in Campylobacter jejuni and C. coli: resistance mechanisms and trends in human isolates. Emerg. Infect. Dis., 7, 24–34 (2001).

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