Affiliation:
1. Max Planck Institute of Colloids and Interfaces, Science Park Golm, 14424 Potsdam, Germany;
Abstract
Giant unilamellar vesicles represent a promising and extremely useful model biomembrane system for systematic measurements of mechanical, thermodynamic, electrical, and rheological properties of lipid bilayers as a function of membrane composition, surrounding media, and temperature. The most important advantage of giant vesicles over other model membrane systems is that the membrane responses to external factors such as ions, (macro)molecules, hydrodynamic flows, or electromagnetic fields can be directly observed under the microscope. Here, we briefly review approaches for giant vesicle preparation and describe several assays used for deducing the membrane phase state and measuring a number of material properties, with further emphasis on membrane reshaping and curvature.
Subject
Cell Biology,Biochemistry,Bioengineering,Structural Biology,Biophysics
Cited by
96 articles.
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