Better, Faster, Cheaper: Recent Advances in Cryo–Electron Microscopy-Reference-Cited by-同舟云学术

Better, Faster, Cheaper: Recent Advances in Cryo–Electron Microscopy

Published:2022-06-21 Issue:1 Volume:91 Page:1-32
ISSN:0066-4154
Container-title:Annual Review of Biochemistry
language:en
Short-container-title:Annu. Rev. Biochem.

Author:

Chua Eugene Y.D.¹²³,Mendez Joshua H.¹²³,Rapp Micah¹²,Ilca Serban L.¹²,Tan Yong Zi⁴⁵,Maruthi Kashyap¹²⁶,Kuang Huihui¹²⁶,Zimanyi Christina M.¹²³,Cheng Anchi¹²⁶,Eng Edward T.¹²³,Noble Alex J.¹²⁶⁷⁸,Potter Clinton S.¹²³⁶⁷,Carragher Bridget¹²³⁶⁷

Affiliation:

1. New York Structural Biology Center, New York, NY, USA;, , , , , , , , , , ,

2. Simons Electron Microscopy Center, New York, NY, USA

3. National Center for CryoEM Access and Training, New York, NY, USA

4. Department of Biological Sciences, National University of Singapore, Singapore;

5. Disease Intervention Technology Laboratory, Agency for Science, Technology and Research (A*STAR), Singapore

6. National Resource for Automated Molecular Microscopy, New York, NY, USA

7. National Center for In-Situ Tomographic Ultramicroscopy, New York, NY, USA

8. Simons Machine Learning Center, New York, NY, USA

Abstract

Cryo–electron microscopy (cryo-EM) continues its remarkable growth as a method for visualizing biological objects, which has been driven by advances across the entire pipeline. Developments in both single-particle analysis and in situ tomography have enabled more structures to be imaged and determined to better resolutions, at faster speeds, and with more scientists having improved access. This review highlights recent advances at each stageof the cryo-EM pipeline and provides examples of how these techniques have been used to investigate real-world problems, including antibody development against the SARS-CoV-2 spike during the recent COVID-19 pandemic.

Publisher

Annual Reviews

Subject

Biochemistry

Link

https://www.annualreviews.org/doi/pdf/10.1146/annurev-biochem-032620-110705

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