Noncontact Nanoscale Imaging of Cells

Author:

Klenerman David1,Korchev Yuri23,Novak Pavel24,Shevchuk Andrew2

Affiliation:

1. Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom;

2. Imperial College Faculty of Medicine, London Centre for Nanotechnology, London W12 0NN, United Kingdom

3. Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan

4. National University of Science and Technology (MISiS), Moscow 119991, Russia

Abstract

The reduction in ion current as a fine pipette approaches a cell surface allows the cell surface topography to be imaged, with nanoscale resolution, without contact with the delicate cell surface. A variety of different methods have been developed and refined to scan the topography of the dynamic cell surface at high resolution and speed. Measurement of cell topography can be complemented by performing local probing or mapping of the cell surface using the same pipette. This can be done by performing single-channel recording, applying force, delivering agonists, using pipettes fabricated to contain an electrochemical probe, or combining with fluorescence imaging. These methods in combination have great potential to image and map the surface of live cells at the nanoscale.

Publisher

Annual Reviews

Subject

Analytical Chemistry

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