Diversity analysis in snowball cauliflower (Brassica oleracea var. botrytis) using microsatellite markers

Abstract

A set of 50 microsatellite markers were used for analysis of molecular diversity in 12 parental lines consisting of five Ogura CMS lines and 7 testers of snowball cauliflower (Brassica oleracea vas botrytis L.). Twelve markers were found to be polymorphic and the polymorphic information content (PIC) of the simple sequence repeat (SSR) markers ranged from 0.15 to as high as 0.83. The genetic dissimilarity based on simple matching coefficient for 12 genotypes ranged from 0.06 to 0.61 with an average of 0.36. The principal coordinate analysis based on molecular polymorphism, delineated the genotypes into five different groups. Genotype specific alleles was identified, which can be used as a reference for varietal identification as well as to identify the purity of the hybrid seeds in snowball cauliflower. The present study demonstrates the utility of microsatellite markers for estimating molecular diversity as well as genotype identification in snowball cauliflower.