Author:
PANWAR SAPNA,SINGH KANWAR PAL,JANAKIRAM T,BHARADWAJ C,NAMITA NAMITA,SONAH HUMA,DESHMUKH R K,SHARMA T R
Abstract
The present investigation was carried out to characterize 22 genotypes of marigold (Tagetes erecta L.) with RAPD (randomly amplified polymorphic DNA) markers. A total of 26 RAPD markers were utilized for assessment of genetic divergence in marigold which amplified a total of 241 amplicons, out of which 238 (98.80%) were found polymorphic. The average number of amplicon per primer was 9.27. The Jaccard's similarity coefficient ranged from0.18 to 0.80. The mean values of polymorphic information content (PIC), resolving power (RP) and marker index (MI) of RAPD primers were 0.38, 8.36 and 3.44 respectively. Cluster analysis was done by unweighted pair group method for arithmetic average (UPGMA) and Principal Component analysis (PCA) was also done for the grouping of genotypes. The broad range of Jaccard's similarity coefficient observed for the genotypic data generated suggest the wide genetic base for marigold genotypes which will be effectively explored for future breeding programmes.
Publisher
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Subject
Agronomy and Crop Science
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