Author:
NARNAWARE SHIRISH DADARAO,TRIPATHI BHUPENDRA NATH
Abstract
Serum samples (531) collected randomly from cattle of organized (200) and unorganized (331) farms of Central and Northern parts of India were subjected to a commercial ELISA to know the seroprevalence of paratuberculosis. These serum samples were also tested by an in-house absorbed ELISA developed in the laboratory and the results were compared. The overall seroprevalence of paratuberculosis in cattle was 8.09% with significantly higher prevalence in cattle of organized farms (13.5%) than unorganized farms (4.83%). The seroprevalence was also significantly higher in calves (17.24%) than adults (7.57%); whereas there was no significant difference in prevalence rate among male (6.71%) and female (9.87%) cattle. Region wise the seroprevalence was slightly higher in organized farms of Northern India (16.43%) than that of Central India (11.81%). The sensitivity and specificity of in-house ELISA were 71.11% and 98.76%, respectively, with the accuracy of over 96%. On the basis of Kappa-test, the inhouse ELISA was in good agreement with commercial Pourquier ® ELISA and can be recommended for screening of paratuberculosis infection in cattle in India.
Publisher
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Subject
General Veterinary,Animal Science and Zoology
Reference21 articles.
1. Behr M A and Collins D M. 2010. Paratuberculosis: Organism, Disease, Control. CAB International Publication, Oxfordshire, UK.
2. Didugu H, Ramanipushpa R N, Narasimha Reddy C H E, Anitha Devi M and Bhaskraramarajusagi S. 2015. Mycobacterium avium subspecies paratuberculosis among bovines in Krishna district of Andhra Pradesh, India: a serological survey. Indian Journal of Animal Health 54: 149–54.
3. Dieguez F J, Arnaiz I, Sanjuan M L, Vilar M J and Yus E. 2008. Management practices associated with Mycobacterium avium subspecies paratuberculosis infection and the effects of the infection on dairy herds. Veterinary Record 162: 614–17.
4. Ferreira R, Fonseca L and Lilenbaum W. 2002. Comparison between a commercial and an in-house ELISA for anti-M. avium paratuberculosis antibodies detection in dairy herds in Rio de Janeiro, Brazil. Revista Latinoamericana de Microbiología 44: 129–32.
5. Garg R, Patil P K, Singh S V, Sharma S, Gandham R K, Singh A V, Filia G, Singh P K, Jayaraman S, Gupta S, Chaubey K K, Tiwari R, Saminathan M, Dhama K and Sohal J S. 2015. Comparative evaluation of different test combinations for diagnosis of Mycobacterium avium subspecies paratuberculosis infecting dairy herds in India. BioMed Research International 2015: doi:10.1155/2015/983978.