Author:
TEJPAL TEJPAL,MEHTA J S,RAVI S K,TALLURI T R,KUMAR ASHOK,KUMAR AMIT,SONI YOGESH
Abstract
Extenders (3) were compared for frozen-thawed semen quality of Marwari horses in the present study. Ejaculates (36) including 6 ejaculates from each of 6 stallions were collected using artificial vagina (AV) method. The ejaculates were cryopreserved either with glucose-EDTA-lactose, HF-20 or egg yolk-skim milk extender using custom freezing technique. Sperm characteristics, viz. motility, viability, acrosome intactness and plasma membrane integrity were studied in frozen-thawed semen besides record of fresh semen quality as a prerequisite. Present study indicated a non-significant difference in frozen-thawed semen parameters with glucose-EDTA-lactose and HF-20 extenders, however, both the extenders resulted in a higher percentage of post-thaw motility (PTM), viability, intact acrosome and plasma membrane integrity of sperm than the egg yolk-skim milk extender. It was concluded that glucose-EDTA-lactose and HF-20 are better semen extenders as compared to egg yolk-skim milk and they can be used effectively for semen cryopreservation in horses.
Publisher
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Subject
General Veterinary,Animal Science and Zoology
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